Dr Alasdair Hubbard

Postdoctoral Research Associate

Graduated with a BSc (Hons) in Microbiology from University of Leeds in 2007 and a Ph.D from St George’s, University of London in 2015. Between BSc and Ph.D, worked at the Health Protection Agency and Dstl, Porton Down.

Post Ph.D worked as a Postdoctoral Scientist at a molecular diagnostic company and a Research Assistant at University of Oxford

Research:

My main research interests are based around the use of genomics and experimental evolution to understand the development of antimicrobial resistance in clinically relevant bacteria. I also have an interest in understanding and characterising novel mechanisms of antimicrobial resistance and early stage drug discovery.

Main projects:

Fitness costs of acquired AMR in Enterobacteriaceae: A reduction in the fitness of bacteria has been found to be associated with acquisition of resistance compared to the parent antimicrobial sensitive strain when the antimicrobial is no longer present. We want to take advantage of this fitness cost to minimise the persistence of resistance within the clinical setting and inform clinicians of the optimal treatment options to slow the emergence and spread of AMR.

Discovery of antimicrobial proteins from snake venom: Proteins with antimicrobial properties have been discovered in a wide range of venom. Using an iterative assay, we are screening snake venom from species of snake housed at LSTM to discover proteins with previously unknown antibacterial activity towards Escherichia coli.

 

Selected publications

  • Hubbard ATM, Mason J, Roberts P, Parry CM, Corless C, Van Aartsen J, Howard A, Bulgasim I, Fraser AJ, Adams ER, et al. Piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of blaTEM-1B. Nat Commun. 2020. 11 4915 10.1038/s41467-020-18668-2

    Hubbard ATM, Bulgasim I and Roberts AP. A novel hemA mutation is responsible for a small-colony-variant phenotype in Escherichia coli. Microbiology. 2020. 1-8 10.1099/mic.0.000962

    Hubbard ATM, Newire E, Botelho J, Reine J, Wright E, Murphy EA, Hutton W and Roberts AP. Isolation of an antimicrobial-resistant, biofilm-forming, Klebsiella grimontii isolate from a reusable water bottle. Microbiologyopen. 2020. 9 1128-1134 10.1002/mbo3.1023

    Nikolaou E, Hubbard ATM, Botelho J, Marschall TaM, Ferreira DM and Roberts AP. Antibiotic Resistance Is Associated with Integrative and Conjugative Elements and Genomic Islands in Naturally Circulating Streptococcus pneumoniae Isolates from Adults in Liverpool, UK. Genes. 2020. 11 2-9 10.3390/genes11060625

    De Maio N, Shaw LP, Hubbard ATM, George S, Sanderson ND, Swann J, Wick R, Abuoun M, Stubberfield E, Hoosdally SJ, et al. Comparison of long-read sequencing technologies in the hybrid assembly of complex bacterial genomes. Microbial Genomics. 2019. 5 1-12 10.1099/mgen.0.000294

    Gweon HS, Shaw LP, Swann J, De Maio N, Abuoun M, Niehus R, Hubbard ATM, Bowes MJ, Bailey MJ, Peto TEA, et al. The impact of sequencing depth on the inferred taxonomic composition and AMR gene content of metagenomic samples. Environmental Microbiome. 2019. 14 1-15 10.1186/s40793-019-0347-1

    Hong WD, Benayoud F, Nixon GL, Ford L, Johnston KL, Clare RH, Cassidy A, Cook DaN, Siu A, Shiotani M, et al. AWZ1066S, a highly specific anti-Wolbachia drug candidate for a short-course treatment of filariasis. Proceedings of the National Academy of Sciences of the United States of America. 2019. 116 1414-1419 10.1073/pnas.1816585116

    Hubbard ATM, Jafari NV, Feasey N, Rohn JL and Roberts AP. Effect of Environment on the Evolutionary Trajectories and Growth Characteristics of Antibiotic-Resistant Escherichia coli Mutants. Frontiers in Microbiology. 2019. 10 1-13 10.3389/fmicb.2019.02001

    Rehal R, Gaffney PRJ, Hubbard ATM, Barker RD and Harvey RD. The pH-dependence of lipid-mediated antimicrobial peptide resistance in a model staphylococcal plasma membrane: A two-for-one mechanism of epithelial defence circumvention. European Journal of Pharmaceutical Sciences. 2019. 128 43-53 10.1016/j.ejps.2018.11.017

    Reynolds ME, Phan HTT, George S, Hubbard ATM, Stoesser N, Maciuca IE, Crook DW and Timofte D. Occurrence and characterization of Escherichia coli ST410 co-harbouring blaNDM-5, blaCMY-42 and blaTEM-190 in a dog from the UK. Journal of Antimicrobial Chemotherapy. 2019. 74 1207-1211 10.1093/jac/dkz017

    Rooney CM, Sheppard AE, Clark E, Davies K, Hubbard ATM, Sebra R, Crook DW, Walker AS, Wilcox MH and Chilton CH. Dissemination of multiple carbapenem resistance genes in an in vitro gut model simulating the human colon. Journal of Antimicrobial Chemotherapy. 2019. 74 1876-1883 10.1093/jac/dkz106

    Hubbard ATM, Davies SEW, Baxter L, Thompson S, Collery MM, Hand DC, Thomas DJI and Fink CG. Comparison of the first whole genome sequence of 'Haemophilus quentini' with two new strains of 'Haemophilus quentini' and other species of Haemophilus. Genome. 2018. 61 379-385 10.1139/gen-2017-0195

    Phan HTT, Stoesser N, Maciuca IE, Toma F, Szekely E, Flonta M, Hubbard ATM, Pankhurst L, Do T, Peto TEA, et al. Illumina short-read and MinION long-read WGS to characterize the molecular epidemiology of an NDM-1 Serratia marcescens outbreak in Romania. Journal of Antimicrobial Chemotherapy. 2018. 73 672-679 10.1093/jac/dkx456

    George, Sophie et al. Resolving plasmid structures in Enterobacteriaceae using the MinION nanopore sequencer: assessment of MinION and MinION/Illumina hybrid data assembly approaches. Microbial genomics vol. 3,8 e000118. 9 Jun. 2017, doi:10.1099/mgen.0.000118